Environmental Wastewater Air Treatment Expert Witness

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Environmental Wastewater Air Treatment Expert Witness

Provides Opinion & Testimony In:

Environmental Wastewater Air Treatment, environmental, wastewater, air treatment, biological, biochemistry, enzymology, biotechnology, physical biochemistry, protein biochemistry, protein characterization, protein modification, protein stabilization, protein structures,enzymes, inter partes review,

EXECUTIVE SUMMARY

Senior consultant, domestic and international, in the following major areas:

Classical chemistry, biochemistry and biotechnology, especially the separation, purification,
characterization and analysis of small molecules, enzymes and proteins and related processes. Adept
at assessing and evaluating current processes and procedures!
Chemical and biochemical processes, their development, evaluation, optimization, including
biocatalysis and applied biotechnology
Environmental science, especially bioremediation and related treatment chemistries and
analysis
Microbiology & fermentation science
Regulatory compliance (OSHA, EPA, Groundwater, USDA, Food Safety, Homeland Security,
etc.)
Expert witness & consultant; patent infringement, claim construction, trade secrets · Inter
partes review

LABORATORY AND APPLIED CHEMISTRY, PROCESSES, ANALYSIS AND SEPARATIONS
SCIENCE:

Broad experience and understanding of physical, chemical and biochemical processes and related analytical
methods (assay development) and: spectroscopy, electrophoretic and chromatographic separations, protein
modification, ligand binding, enzyme assay and kinetics, thermodynamic calculations, polymer chemistry and
polymerization processes, etc.

Analytical chemistry
Bioassays and separations science (especially chromatography, liquid-liquid extractions, countercurrent
separations; electrophoresis)

Environmental
Biochemistry and biophysics combined with physical chemistry, especially through the use of UV/Vis
(Ultraviolet / Visible) Spectrophotometry
Small molecule binding studies, ligands, inhibitors especially involving enzymes
Separations technology for purification and analysis of numerous small molecules, proteins, peptides,
carbohydrates, and natural products used in commercial applications; adsorbents, ion exchange and activated
carbon

Separations science experience includes extensive application of:
Chromatographic separations (column chromatography, liquid chromatography): proteins, enzymes,
small molecules
DEAE cellulose
CM cellulose
DEAE Sephadex
CM Sephadex
CG-50
Hydrophobic interaction chromatography (HIC)
Sepharose
Size exclusion (Sephadex)
Hydroxyapatite
Centrifugal separations
HPLC (reverse phase, etc.)
Ion chromatography
Detectors (esp. UV/Vis)
SORBEX: Industrial-Scale Adsorptive Separation
Supervisory experience with GC-MS and LC-MS.
TLC (small molecules, e.g., hemes, dyes, aminacids)
Alumina
Silica
Polyamide
Electrophoresis
Polyacrylamide gels (PAGE)
SDS
Cellulose acetate
pH-stat and autotitrator operation (enzyme assay)
Membrane separations
Reverse Osmosis
Ultrafiltration
Microfiltration
Liquid/liquid, liquid/solid separations, supercritical fluid extractions
Enzyme purification via precipitative processes using…
Chaotropic agents (altering solubility, salting out, precipitations)
Salts, esp. (NH4)2SO4
Organic solvents (acetone, ethanol)
Polymers
Polymerization process
Polyimines, acrylates and methacrylates; cellulose acetate, starch dialdehyde, etc
Conjugation, crosslinking, derivatization and surface application of monomers and polymers
Surface modification
Functionalized silanes for surface modification3
PEI/Glutaraldehyde

PROCESS EVALUATION

Adept at evaluating current processes at customer facilities in the Chemical, Biochemical,
Microbiological and Food industries and suggested improvements tincrease efficiency and reduce
costs.

CHEMISTRY and BIOTECHNOLOGY

Expert in applied industrial chemistry, enzymology, biocatalysis, biotransformations & bioprocessing,
especially low-waste “green” systems and thermostable enzymes; emphasis on practical aspects of research,
development, scale-up, pilot plant and commercialization. Adept at working with both laboratory personnel
& development engineers; hands on when needed.
Applied enzymology: Marriage of organic chemistry and enzymology… use of enzymes tmake
products in high yield with little waste (see examples below).
Industrial enzyme projects include mostly “green” and thermostable industrial processes; Expert in
continuous, heterogeneous biologically catalyzed processes (immobilized enzymes and immobilized
whole cells).
Preparation and application of immobilized whole cell hydantoinase suite of enzymes for industrial
biotransformation process for production of enantiomerically-pure aminacids
Pilot-scale immobilization of pig liver esterase (ImPLE) for production of anti-AIDS drug and subsequent
project management for scale-up
Development of full-scale commercial process for ImPLE (above); implementation and supervision of
production, quality control and international shipping
Employing a statistical DOE approach for optimizing coupling of a peptide with a microbial polysaccharide for
commercial vaccine production (Tetanus Toxoid)
Employing a statistical DOE approach for optimization of conditions for efficiently coupling of enzyme to
chemically-modified polymer surface
Optimization of ethanol precipitation of plasma proteins for industrial plasma fractionation
Acetone precipitation and purification of proteins
Use of salts for commercial enzyme/protein purification
Development and testing of novel hydrophobic enzyme support for industrial lipase, esterase application in
production of pharmaceuticals
Fermentation, E. coli producer strain, optimization of cell yield
Immobilization and application of genetically modified Pseudomonas sp lipase for enantioselective hydrolysis
of pharmaceutical intermediates
Research and commercial development of novel, incompressible, high-throughput immobilized
enzymes (food industry).
Invention, application, and development of new method for enzyme stabilization applied tstarch
and sugar converting enzymes (glucoamylase, alpha amylase) for corn syrup production
Invention of novel magnetic support for enzyme immobilization
Commercial application of thermodynamics of activation vs. deactivation (Arrhenius plot) to
increase industrial enzyme process efficiency by 20-30%
Other industrial bioprocessing, enzyme transformation and biochemical projects include:
Purification and characterization of numerous enzymes, especially those relating tcarbohydrate
conversions
Chemical modification of hydrocolloids for metal binding application (food and environmental
applications)
Hydrocolloids for enzyme immobilization (food application)
Lipases for making synthetic cocoa butter
Lipases for mono- and diglyceride production and transesterification intvalue added products
(especially involving ferulic acid)
Lipases (Cal B; thermostable) for transesterification of ferulate for increased lipophilicity
(topically-applied sunscreen and skin care consumer product application), especially employing
soy/soya lipids/oils and fish oils.
Lactase /protease combination employed for whey treatment
Yeast alcohol oxidase for methanol oxidation
Horseradish peroxidase for enzyme stabilization
Glucose oxidase for production of D-glucono-δ-lactone
Glutaraldehyde as sterilant for food applications
Biochemical fuel cell
Microbiology, especially fermentative production of enzymes
Pharmaceutical industry: Use of applied enzymology for the production of pharmaceutical
intermediates; patent holder, peptide-based anti-microbial pharmaceuticals employed in wound dressing
applications.
Commercialization: Multiple patent holder, biologically-based reactor development and
commercialization (especially fixed-film reactors).

ENVIRONMENTAL CHEMISTRY and BIOTECHNOLOGY, INDUSTRIAL EFFLUENT
PRETREATMENT, BIOREMEDIATION AND EMISSIONS TREATMENT

Nationally recognized consultant in biological and chemical treatment of hazardous waste, hazardous
chemicals, water pollution, drinking/potable water
Microbial ecology approach twaste treatment and bioremediation
Extensive field experience… pilot plant direction, data analysis, scale-up estimation
Developed structured adsorbent used as microbial support in bioremediation of industrial effluent,
groundwater and vaporous emissions (patented/commercial)
Remediation and bioremediation of wide range of pollutants, including petroleum, coal tar, halogenated,
chlorinated (esp. TCE, PCBs), aromatic compounds
Developed mixed media employed as plugging-free microbial support in bioreactor packing
(patented/commercial)
Developed in situ-ex situ bioremediation method for detoxification of metals, especially hexavalent
chromium, Cr(VI), by combining inorganic chemistry and microbial ecology (sulfate reducing bacterial
consortia); patented
Chemically modified natural polymers for use as heavy metal adsorbers.
Developed Biological Air Treater (BAT) for low-cost destruction of vaporous organics, air pollution
(patented/commercial)
On-Site international experience (China [PRC], Taiwan [ROC], UK)
Designed and implemented numerous waste treatment and remediation strategies for diverse industrial
effluents, impacted groundwaters, drinking water, soils, and air emissions
Supervised replacement of PCB (polychlorinated biphenyl) -Transformer and replaced with non-toxic,
biodegradable electrolyte-containing transformer

FOOD AND NUTRITIONAL SUPPLEMENTS

Starches and starch metabolizing enzymes (amylases)5
HFCS
Fish oil and Omega-3 fatty acids
Patent infringement involving DHA purification and analysis
New product technology (transesterification) involving vegetable oils, fish oils (triacylglycerides)
and anti-oxidants
Vitamins, especially B vitamin protection against reperfusion injury (ischemic stroke and heart attack)
and anti-inflammatory effects of riboflavin (vitamin b2)

FOOD SAFETY USDA AND FDA

Food storage and handling
Odor reduction and sanitation using chlorinated chemicals
HACCP plans (Hazard Analysis and Critical Control Point) and process flow charts, especially for
ready teat foods [9cfr PART 417]
SSOP (Sanitation Standard Operating Procedures); Regulatory requirements under the federal meat
inspection act and the poultry products inspection act, especially 9cfr PART 416—SANITATION)

REGULATORY, OSHA & EPA

Multiple private consulting projects tachieve compliance with Federal & State regulations, including:
OSHA 1910.119, Process Safety Management (PSM)
EPA 112r, Risk Management Planning (RMP);
SARA Title III Emergency Planning and Community Right tKnow Act (EPCRA);
TSCA (Toxic Substances Control Act) 40cfr §761 Polychlorinated Biphenyls (PCBs)
Storm Water Pollution Prevention Plans (SWPPP).
Drinking water standards

TECHNICAL MARKETING, SALES, MANAGERIAL & BUSINESS
ACCOMPLISHMENTS & EXPERIENCE

Experienced in all phases of commercialization business, from research tdevelopment tmarketing and
sales;
Successfully commercialized laboratory scale reactor (biological waste treatment) through rapid
development phase intcommercial product; business profitable at 13 months; ten-fold increase in sales.
Account manager, proposal generation, expert in managing numerous $100K t$1000K projects from
conception tcommercialization.
Budgetary estimation.
Technical sales presentations directly tcustomers; responsible for visiting outside customers to
determine their actual needs and then partnering/ bridging with internal experts tachieve technical and
commercial goals.
Technical marketing and sales, environmental area.
Excellent communication skills.
Professional corporate recruiting (fourteen years).
Long-term experience in TQ approach tsolving cross-disciplinary problems with teams of 2 t10
individuals, on and off site.

EXPERT WITNESS and PATENT INFRINGEMENT

Insurance investigations
Toxic tort
Root cause analysis
Patent infringement
Claim construction
Deposition
Preparation for trial
Evidentiary hearing
Jury trial experience
Inter partes review
Trade secrets
Litigation-related experience supplied upon request

EDUCATION

Postdoctoral Fellow under Prof. Quentin H. Gibson, Cornell University, 7/76 – 7/78.
Postdoctoral Fellow under Prof. Donald E. Hultquist, The University of Michigan, 1/76 – 6/76.
Ph.D., Biological Chemistry, The University of Michigan, 9/71 – 1/76.
BA, Chemistry (Honors), Queens College, The City University of New York 5/71.

SELECT TRAINING/DEVELOPMENT/PRESENTATIONS

HAZWOPPER Emergency response Refresher Training (40 hour initial training, 8 hour Haz. Waste
Operations Management continuing yearly), 1991 tCurrent (2016).
Emergency Notification Release Reporting Training, EPCRA 304; CERCLA 103 (EPA Region 5),
1999
Risk Management Program Assistance (IEMA), 1999
Basic Ammonia Refrigeration, 1998
Statistical Design of Experiments (DOE), 1996
Scorecard Training, 1996
Convenor, Pollution Prevention Biotechnology, Summer Biotechnology Institute, Northwestern
University Center for Biotechnology, 1995
Sales Training Workshop (Institute for Business and Interpersonal Strategies) 1995
Customer Linked Commercialization & Customer Partnering, 1994

EMPLOYMENT HISTORY

Industrial
Independent Consultant, President and Member, , 1999 tpresent
Independent Consultant, Sole proprietorship, 1995 t1996 and 1997 t1999
Project Leader, AlliedSignal Aerospace Sector, Extended Consultation Assignment (Space Station /
Mars Lander / Lunar Base), Life Support Systems, ‘96 t’97; manager biotech and environmental
laboratory.
Manager Biochemical Technology (Technical Marketing and Sales), AlliedSignal Environmental
Systems and Services, ‘94-‘95
Research Scientist, AlliedSignal Research & Technology, ‘85-‘94
Research Specialist, Biochemistry, Signal-UOP Research Center, ‘82-‘85
Senior Research Biochemist, Biotechnology Department, UOP Research Center, ‘78-‘82
Academic
Adjunct Professor (Evening Faculty Member), Columbia College, Dept. of Science, ‘84-‘85.
Postdoctoral and Teaching Fellow (General biochemistry), Cornell University, Department of
Biochemistry, Molecular and Cell Biology, ‘77-‘78.
Teaching Assistant (General biochemistry, Medical Technology & Nursing) The University of
Michigan, ‘71-‘75.

HONORS

Special (Team) Recognition Award, AlliedSignal (Honeywell), April ‘95
Special (Individual) Recognition Award, AlliedSignal (Honeywell), May ‘92
Rackham Dissertation Grant, The University of Michigan, ‘74-‘75
Graduated (BA, Queens College) with honors, ‘71
Elected tBeta Delta Chi, Chemistry Honor Society, Queens College, CUNY, ‘71
New York State Regents Scholarship, ‘67-‘71

SOCIETY MEMBERSHIPS, ELECTED AND APPOINTED POSITIONS

American Chemical Society (elected Director, ChicagSection, 2014 for twyear term)
Appointed Trustee by Cook County Commissioner, Plum Grove Estates Sanitary District, 2005 to
present (2016)
Water Environment Federation

PATENTS AND PUBLICATIONS

Authored approximately 22 publications, 3 book chapters, and is an inventor on approximately 15 issued
patents, in biotechnology, chemical, food, pharmaceutical industries, and biological wastewater treatment
areas; co-Editor, John Wiley & Sons, “Biological Treatment of Hazardous Wastes” 1998.

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